Regulatory

Part:BBa_K1315001:Experience

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-20)


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Applications of BBa_K1315001

User Reviews

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iGEM Dundee 2014

This pqsA promoter region was hooked up to an mCherry gene from Dundee iGEM 2011 BBa_K562011. This was then co-expressed with Pseudomonas aeruginosa PqsR from BBa_K1157001. In an E. coli chassis the device was exposed to commercially-available PQS, grown for several hours, then immunoblotted for mCherry production (Dundee Figure 1). The device did not produce enough mCherry for detection using this method.

Dundee 2014 Figure 1 Western blot of (no) production of mCherry from a pqsA promoter co-expressed with PqrR

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